Dexamethasone inhibits U937 cell adhesion via the down-regulation of ROCK1 activity.
نویسندگان
چکیده
OBJECTIVE To explore the effect of dexamethasone (DEX) on monocyte adhesion function and its underlying mechanism. METHODS The effects of DEX and fasudil on adhesion of cultured U937 monocytes to human umbilical vein endothelial cells (HUVEC) following stimulation with phorbol myristate acetate (PMA) were studied; Changes in the Rho-associated coiled-coil protein kinase 1 (ROCK1) protein content and activity were evaluated. RESULTS DEX and fasudil significantly inhibited U937 cell adhesion rates under PMA stimulation and inhibited ROCK1 activity. Mifepristone (RU-486) and cycloheximide (CHX) did not alter these effects of DEX. CONCLUSIONS DEX interferes with the adhesion function of U937 cells through the inhibition of ROCK1 activity.
منابع مشابه
The glucocorticoid dexamethasone inhibits U937 cell adhesion and neutrophil release via RhoA/ROCK1-dependent and independent pathways.
AIMS The aim of the present study was to investigate the role of the Ras homolog family member A (RhoA)/Rho-associated coiled-coil-containing protein kinase 1 (ROCK1) signaling pathway in the inhibition of inflammatory responses by the glucocorticoid dexamethasone (Dex). METHODS The inhibitory effects of Dex and Rho-kinase inhibitor fasudil (Fas) on phorbol ester-induced release of O2(-) and ...
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ورودعنوان ژورنال:
- Acta biochimica Polonica
دوره 59 4 شماره
صفحات -
تاریخ انتشار 2012